Coventry City travel to the Weston Homes Community Stadium on Tuesday night for the first ever time.
However, this will be the 28th match between the two sides with City winning thirteen and drawing seven, scoring 42 goals to Colchester's 31.
City's last visit to Colchester saw a 5-1 win at Layer Road on Saturday April 19th 2008. Scott Vernon fired Colchester ahead in the 16th minute but the Sky Blues hit back in the second half with goals from Leon Best, Danny Fox, Michael Doyle and two Elliott Ward penalties.
The teams that day were:
City: Schmeichel, Fox, Ward, Dann, Osbourne (Hall 92), Doyle, M.Hughes, S.Hughes, Mifsud, Best (Hines 86), Tabb SNU Thornton, Marshall, Gray
Gerken,Ifill,Coyne,Balogh,White (McLeod 71), Duguid, Hammond, Jackson, Ellio, Lisbie, Vernon SNU Bankole,Virgo, Izzet,Platt
That was the fourth win for City in Colchester from 13 meetings with three draws.